2v3f

ACID-BETA-GLUCOSIDASE PRODUCED IN CARROT CELLS
(see also Treatment of Gaucher disease)

 Three-dimensional structure of recombinant plant-derived glucocerebrosidase (prGCD) consists of 3 domains. Domain I (residues 1–27 and 384–414, colored pink ) comprises a 3-stranded anti-parallel β-sheet flanked by a perpendicular amino-terminal strand. Domain II (residues 30–75 and 431–497, colored lime) consists of two β-sheets. Domain III (residues 76–381 and 416–430, colored red) is a (β/α)8 TIM barrel. The catalytic site with molecule BTB is shown.

Structural alignment of prGCD with both Cerezyme® (1ogs) and Cerezyme® covalently modified by an irreversible inhibitor, conduritol-B-epoxide (1y7v, colored yellow ), revealed highly significant structural identity. The RMSD values for Cα atoms of these structures were of 0.64 and 0.60 Å, respectively. Moreover, there was strict conservation of the active site residues. 

About this Structure
2V3F is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference
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